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Creatinine levels in urine can be used as an indicator of proper kidney function and a reference to normalize other tests. Reliable serum creatinine measurements in glomerular filtration rate estimation are critical in the diagnosis and treatment of chronic kidney disease.

Serum and urine creatinine has been analyzed by alkaline picrate methods, enzymatic or partially enzymatic assays, and HPLC methods. Analysis of serum creatinine by the alkaline picrate methods are interfered by many endogenous and exogenous substances, leading to the overestimation of serum creatinine, especially at low serum creatinine levels. Though there are fewer interfering substances in enzymatic assays, they still lack analytical specificity in certain applications. Interferences in HPLC methods were also observed. Overall, there are still many challenges in analytical specificity and sensitivity for creatinine measurements in certain applications, e.g., the analysis of mouse serum creatinine, which is quite important due to the fact that more and more mouse models of human diseases have been generated.

GC-IDMS is considered the method of choice for establishing the true concentration of creatinine in serum because of its excellent specificity and relative SD. Derivatization is required in GC/IDMS methods, however. In addition, samples have to be purified by ion-exchange to remove creatine, which derivatized into the same chemical species as creatinine.

We provides LC-MS/MS based highly selective, sensitive, and accurate analysis of creatinine with deuterated creatinine as internal standard:

  • Quantification limit of 0.01 mg/dL (0.88 Ámol/L)
  • Plasma, serum, and urine samples
  • Quick turnaround
  • Method development for a lower quantification limit and other biological fluid or tissue samples upon request