Bioanalytical CRO Service Provider
DMPK Assay CRO Service Provider
ADME CRO Assay Service Provider
Biomarker Analysis CRO Service Provider
CMC Analytical Stability Testing CRO Service Provider
Most drugs are more or less bound to proteins. Basic drugs tend to bind the acidic alpha-1 acid glycoprotein (AAG), while acidic and neutral drugs tend to bind albumin, which is basic. Extent of plasma protein binding may significantly influence the distribution, clearance, and efficacy of a drug.
Our plasma protein binding assay is based on equilibrium dialysis.
After incubation, both the plasma and buffer samples are analyzed by LC-MS/MS for drug concentration. The percent protein binding of a drug is then calculated:
% Protein Binding = 100 * (Cplasma - Cbuffer) / Cplasma
Warfarin and propranolol are used as controls in our protein binding assay.